3J9P
Structure of the TRPA1 ion channel determined by electron cryo-microscopy
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 7 seconds before plunging. |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 1160 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.24 |
| Software | RELION |
| Symmetry | C4 |
