3J98
Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIa)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 3.5 seconds before plunging. |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.3 |
| Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.4 |
| Software | PHENIX,UCSF Chimera,RELION |
| Symmetry | C1 |
