3ISZ
Crystal structure of mono-zinc form of succinyl-diaminopimelate desuccinylase from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.176, 95.709, 181.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.860 - 2.000 |
| R-factor | 0.20469 |
| Rwork | 0.202 |
| R-free | 0.25444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ic1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.720 |
| Number of reflections | 54213 | |
| <I/σ(I)> | 18.7 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 291 | Ammonium sulfate, 0.2 M NaCl, 0.1 M Na Acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
