3IGQ
Crystal structure of the extracellular domain of a bacterial pentameric ligand-gated ion channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.450, 130.240, 113.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.300 |
R-factor | 0.20902 |
Rwork | 0.207 |
R-free | 0.25515 |
Structure solution method | SIRAS |
RMSD bond length | 0.019 |
RMSD bond angle | 1.831 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.057 | 0.424 |
Number of reflections | 105975 | |
<I/σ(I)> | 11.23 | 1.96 |
Completeness [%] | 98.4 | 97.91 |
Redundancy | 2.03 | 2.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |