3I3A
Structural Basis for the Sugar Nucleotide and Acyl Chain Selectivity of Leptospira interrogans LpxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 108.155, 108.155, 116.886 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.790 - 2.120 |
| R-factor | 0.21585 |
| Rwork | 0.213 |
| R-free | 0.26865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.312 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.790 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 41533 | |
| Completeness [%] | 98.0 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 25-fold molar excess of R-3-hydroxylauroyl-methylphosphopantetheine over LiLpxA was crystallize in 35% w/v PEG 3350 and 0.4 M diammonium citrate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
