3HY6
Structure of human MTHFS with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.511, 145.221, 59.655 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.090 - 2.100 |
R-factor | 0.21728 |
Rwork | 0.215 |
R-free | 0.25527 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hxt |
RMSD bond length | 0.011 |
RMSD bond angle | 1.280 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.550 | 2.100 |
High resolution limit [Å] | 2.100 | 2.000 |
Number of reflections | 12552 | |
Completeness [%] | 99.3 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 100mM HEPES, pH6.6, 20mM MgCl2.6H2O, 20mM NiCl2.6H2O, 20% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |