3HXM
Structure of an argonaute complexed with guide DNA and target RNA duplex containing two mismatches.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.839, 118.867, 59.186 |
| Unit cell angles | 90.00, 114.79, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| Rwork | 0.221 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f73 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.650 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.126 | 0.537 |
| Number of reflections | 27365 | |
| <I/σ(I)> | 18.7 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 308 | pH 6.5, VAPOR DIFFUSION, temperature 308 K |
