3HS0
Cobra Venom Factor (CVF) in complex with human factor B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9834 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 134.026, 136.975, 283.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.883 - 3.000 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hrz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.888 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.091 | 0.555 |
Total number of observations | 58990 | |
Number of reflections | 104868 | |
<I/σ(I)> | 12.6 | 2.8 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | PEG 1500, 2,3 butanediol, malic acid 2-(N-morpholino)ethanesulfonic acid tris (hydroxymethyl)aminomethane buffer (MMT), pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |