3HPG
Visna virus integrase (residues 1-219) in complex with LEDGF IBD: examples of open integrase dimer-dimer interfaces
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9696 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.104, 148.904, 91.076 |
Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
Resolution | 38.672 - 3.280 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2B4J and 1K6Y |
RMSD bond length | 0.011 |
RMSD bond angle | 1.261 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.460 |
High resolution limit [Å] | 3.280 | 3.280 |
Rmerge | 0.110 | 0.665 |
Number of reflections | 34296 | 5069 |
<I/σ(I)> | 2 | |
Completeness [%] | 99.6 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 291 | 25-30% Jeffamine M600, 100mM Bis-Tris propane-HCl, pH6.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |