3HOT
Crystal structure of the Mos1 mariner paired end complex with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.294, 84.989, 132.116 |
| Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
| Resolution | 38.870 - 3.250 |
| R-factor | 0.25062 |
| Rwork | 0.248 |
| R-free | 0.30413 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f7t |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.704 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.173 | 0.445 |
| Number of reflections | 38972 | |
| <I/σ(I)> | 8.2 | 2.5 |
| Completeness [%] | 93.2 | 97.6 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
