3HL2
The crystal structure of the human SepSecS-tRNASec complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-08-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 166.818, 166.818, 236.322 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.950 - 2.810 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bc8 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 89375 | |
| <I/σ(I)> | 6 | 1 |
| Completeness [%] | 97.5 | 94.9 |
| Redundancy | 5.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 285 | 0.3M tri-lithium citrate, 18% (w/v) PEG 3,350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
