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3HL2

The crystal structure of the human SepSecS-tRNASec complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-E
Synchrotron siteAPS
Beamline24-ID-E
Temperature [K]77
Detector technologyCCD
Collection date2008-08-10
DetectorADSC QUANTUM 315
Spacegroup nameP 31 1 2
Unit cell lengths166.818, 166.818, 236.322
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution37.950 - 2.810
R-factor0.203
Rwork0.203
R-free0.23800
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3bc8
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.900
High resolution limit [Å]2.8002.800
Number of reflections89375
<I/σ(I)>61
Completeness [%]97.594.9
Redundancy5.44.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
16.52850.3M tri-lithium citrate, 18% (w/v) PEG 3,350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K

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