3HHQ
Crystal structure of apo dUT1p from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-11 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 |
Unit cell lengths | 95.461, 95.466, 97.594 |
Unit cell angles | 98.79, 97.42, 107.13 |
Refinement procedure
Resolution | 40.620 - 2.000 |
R-factor | 0.17783 |
Rwork | 0.175 |
R-free | 0.23581 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f4f |
RMSD bond length | 0.021 |
RMSD bond angle | 1.851 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.441 |
Number of reflections | 191238 | |
<I/σ(I)> | 19.13 | 3.45 |
Completeness [%] | 89.2 | 88.3 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Ammonium sulfate, 0.1M Bis-Tris pH 5.5, 25% PEG 3350 plus 0.015 mg/mL Trypsin. Cryoprotected with a solution of 7% Glycerol, 7% Ethylene glycol and 7% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 295K |