3HEI
Ligand Recognition by A-Class Eph Receptors: Crystal Structures of the EphA2 Ligand-Binding Domain and the EphA2/ephrin-A1 Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-02 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 58.483, 102.108, 135.255 |
Unit cell angles | 84.25, 79.77, 73.94 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.1749 |
Rwork | 0.172 |
R-free | 0.23299 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 2.049 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.069 | 0.045 | 0.273 |
Number of reflections | 192014 | ||
<I/σ(I)> | 18.214 | ||
Completeness [%] | 96.1 | 99.2 | 91.2 |
Redundancy | 2.3 | 2.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 8,000, 100 mM Tris, pH 8.5, vapor diffusion, hanging drop, temperature 293K |