3HDH
PIG HEART SHORT CHAIN L-3-HYDROXYACYL COA DEHYDROGENASE REVISITED: SEQUENCE ANALYSIS AND CRYSTAL STRUCTURE DETERMINATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 295 |
| Collection date | 1985-01 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 227.200, 82.100, 124.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.223 * |
| Rwork | 0.223 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3had |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.046 * | |
| Number of reflections | 28840 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 98.0 | 85 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | gel filtration * | 8 | 10 MM TRIS, PH 8.0, + 1MM DTT + 11-14% PEG 6K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | 0.05 (%) | ||
| 4 | 1 | drop | protein | 5 (mg/ml) | |
| 5 | 1 | reservoir | PEG6000 | 11-14 (%(w/v)) |
