3HDH
PIG HEART SHORT CHAIN L-3-HYDROXYACYL COA DEHYDROGENASE REVISITED: SEQUENCE ANALYSIS AND CRYSTAL STRUCTURE DETERMINATION
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006631 | biological_process | fatty acid metabolic process |
| A | 0006635 | biological_process | fatty acid beta-oxidation |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0050796 | biological_process | regulation of insulin secretion |
| A | 0070403 | molecular_function | NAD+ binding |
| B | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006631 | biological_process | fatty acid metabolic process |
| B | 0006635 | biological_process | fatty acid beta-oxidation |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0050796 | biological_process | regulation of insulin secretion |
| B | 0070403 | molecular_function | NAD+ binding |
| C | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase activity |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005759 | cellular_component | mitochondrial matrix |
| C | 0006629 | biological_process | lipid metabolic process |
| C | 0006631 | biological_process | fatty acid metabolic process |
| C | 0006635 | biological_process | fatty acid beta-oxidation |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0050796 | biological_process | regulation of insulin secretion |
| C | 0070403 | molecular_function | NAD+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE NAD A 350 |
| Chain | Residue |
| A | LEU25 |
| A | LYS115 |
| A | ASN135 |
| A | SER137 |
| A | HIS158 |
| A | PHE159 |
| A | MET26 |
| A | ASP45 |
| A | GLN46 |
| A | ALA107 |
| A | ILE108 |
| A | VAL109 |
| A | GLU110 |
| A | VAL114 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE NAD B 750 |
| Chain | Residue |
| B | GLY24 |
| B | LEU25 |
| B | MET26 |
| B | ASP45 |
| B | GLN46 |
| B | ALA107 |
| B | ILE108 |
| B | GLU110 |
| B | LYS115 |
| B | ASN135 |
| B | SER137 |
| B | PHE159 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE NAD C 1150 |
| Chain | Residue |
| C | GLY22 |
| C | LEU25 |
| C | MET26 |
| C | ASP45 |
| C | GLN46 |
| C | ALA107 |
| C | ILE108 |
| C | GLU110 |
| C | LYS115 |
| C | LEU118 |
| C | ASN135 |
| C | THR136 |
| C | SER137 |
| C | HIS158 |
| C | PHE159 |
| C | ASN161 |
Functional Information from PROSITE/UniProt
| site_id | PS00067 |
| Number of Residues | 25 |
| Details | 3HCDH 3-hydroxyacyl-CoA dehydrogenase signature. DtpGFIvNRllvPYLieavr.LYerG |
| Chain | Residue | Details |
| A | ASP201-GLY225 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 15 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:3479790 |
| Chain | Residue | Details |
| A | ALA34 | |
| B | THR149 | |
| C | ALA34 | |
| C | GLY57 | |
| C | LEU122 | |
| C | ALA127 | |
| C | THR149 | |
| A | GLY57 | |
| A | LEU122 | |
| A | ALA127 | |
| A | THR149 | |
| B | ALA34 | |
| B | GLY57 | |
| B | LEU122 | |
| B | ALA127 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 9 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:Q16836 |
| Chain | Residue | Details |
| A | ASN73 | |
| A | PHE80 | |
| A | LYS173 | |
| B | ASN73 | |
| B | PHE80 | |
| B | LYS173 | |
| C | ASN73 | |
| C | PHE80 | |
| C | LYS173 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | SITE: Important for catalytic activity => ECO:0000250|UniProtKB:Q16836 |
| Chain | Residue | Details |
| A | GLU170 | |
| B | GLU170 | |
| C | GLU170 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q61425 |
| Chain | Residue | Details |
| A | PHE80 | |
| A | ILE206 | |
| B | PHE80 | |
| B | ILE206 | |
| C | PHE80 | |
| C | ILE206 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 24 |
| Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q61425 |
| Chain | Residue | Details |
| A | VAL81 | |
| B | SER87 | |
| B | THR136 | |
| B | LEU185 | |
| B | LEU192 | |
| B | THR202 | |
| B | VAL212 | |
| B | GLY241 | |
| C | VAL81 | |
| C | SER87 | |
| C | THR136 | |
| A | SER87 | |
| C | LEU185 | |
| C | LEU192 | |
| C | THR202 | |
| C | VAL212 | |
| C | GLY241 | |
| A | THR136 | |
| A | LEU185 | |
| A | LEU192 | |
| A | THR202 | |
| A | VAL212 | |
| A | GLY241 | |
| B | VAL81 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q61425 |
| Chain | Residue | Details |
| A | PHE125 | |
| A | GLN179 | |
| B | PHE125 | |
| B | GLN179 | |
| C | PHE125 | |
| C | GLN179 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 3 |
| Details | MOD_RES: N6-(2-hydroxyisobutyryl)lysine => ECO:0000250|UniProtKB:Q16836 |
| Chain | Residue | Details |
| A | ALA127 | |
| B | ALA127 | |
| C | ALA127 |
