3HDH

PIG HEART SHORT CHAIN L-3-HYDROXYACYL COA DEHYDROGENASE REVISITED: SEQUENCE ANALYSIS AND CRYSTAL STRUCTURE DETERMINATION

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003857	molecular_function	(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0006629	biological_process	lipid metabolic process
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0007283	biological_process	spermatogenesis
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0030154	biological_process	cell differentiation
A	0042802	molecular_function	identical protein binding
A	0050796	biological_process	regulation of insulin secretion
A	0070403	molecular_function	NAD+ binding
A	0120162	biological_process	positive regulation of cold-induced thermogenesis
B	0003857	molecular_function	(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0006629	biological_process	lipid metabolic process
B	0006631	biological_process	fatty acid metabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0007283	biological_process	spermatogenesis
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0030154	biological_process	cell differentiation
B	0042802	molecular_function	identical protein binding
B	0050796	biological_process	regulation of insulin secretion
B	0070403	molecular_function	NAD+ binding
B	0120162	biological_process	positive regulation of cold-induced thermogenesis
C	0003857	molecular_function	(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0006629	biological_process	lipid metabolic process
C	0006631	biological_process	fatty acid metabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0007283	biological_process	spermatogenesis
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0030154	biological_process	cell differentiation
C	0042802	molecular_function	identical protein binding
C	0050796	biological_process	regulation of insulin secretion
C	0070403	molecular_function	NAD+ binding
C	0120162	biological_process	positive regulation of cold-induced thermogenesis

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE NAD A 350

Chain	Residue
A	LEU25
A	LYS115
A	ASN135
A	SER137
A	HIS158
A	PHE159
A	MET26
A	ASP45
A	GLN46
A	ALA107
A	ILE108
A	VAL109
A	GLU110
A	VAL114

---

site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE NAD B 750

Chain	Residue
B	GLY24
B	LEU25
B	MET26
B	ASP45
B	GLN46
B	ALA107
B	ILE108
B	GLU110
B	LYS115
B	ASN135
B	SER137
B	PHE159

---

site_id	AC3
Number of Residues	16
Details	BINDING SITE FOR RESIDUE NAD C 1150

Chain	Residue
C	GLY22
C	LEU25
C	MET26
C	ASP45
C	GLN46
C	ALA107
C	ILE108
C	GLU110
C	LYS115
C	LEU118
C	ASN135
C	THR136
C	SER137
C	HIS158
C	PHE159
C	ASN161

---

Functional Information from PROSITE/UniProt

site_id	PS00067
Number of Residues	25
Details	3HCDH 3-hydroxyacyl-CoA dehydrogenase signature. DtpGFIvNRllvPYLieavr.LYerG

Chain	Residue	Details
A	ASP201-GLY225

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	27
Details	Binding site: {"evidences":[{"source":"PubMed","id":"3479790","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q16836","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Site: {"description":"Important for catalytic activity","evidences":[{"source":"UniProtKB","id":"Q16836","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q61425","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	26
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q61425","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q61425","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	Modified residue: {"description":"N6-(2-hydroxyisobutyryl)lysine","evidences":[{"source":"UniProtKB","id":"Q16836","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 2hdh

Chain	Residue	Details
A	ASN208
A	SER137
A	HIS158

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 2hdh

Chain	Residue	Details
B	ASN208
B	SER137
B	HIS158

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 2hdh

Chain	Residue	Details
C	ASN208
C	SER137
C	HIS158

---