3HAV

Structure of the streptomycin-ATP-APH(2")-IIa ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 31
Unit cell lengths	128.620, 128.620, 57.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.780 - 2.450
R-factor	0.22075
Rwork	0.218
R-free	0.28382
Structure solution method	MIR
Starting model (for MR)	3ham
RMSD bond length	0.011
RMSD bond angle	1.192
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.900
High resolution limit [Å]	2.450
Rmerge	0.043
Number of reflections	41774
<I/σ(I)>	20.1
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	277	30% mPEG5000, 0.2 M MOPS/KOH pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K