3H32
Crystal structure of D-dimer from human fibrin complexed with Gly-His-Arg-Pro-Tyr-amide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 264.720, 97.320, 132.490 |
Unit cell angles | 90.00, 122.78, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.600 |
R-factor | 0.2631 |
Rwork | 0.263 |
R-free | 0.31990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fze |
RMSD bond length | 0.013 |
RMSD bond angle | 1.679 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.600 |
Number of reflections | 31584 |
Completeness [%] | 95.0 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Equal volumes of (a) 5 mg/mL D-dimer, 0.5 mM GHRPYam, 0.3 mM GPRPam, 0.05 M Tris-HCl pH 8.0 and (b) 1% PEG 3350, 1 mM Iodoacetamide, 1 mM CaCl2, 2mM Sodium azide, VAPOR DIFFUSION, SITTING DROP, temperature 295K |