3H2X
Crystal Structure of The Human Lymphoid Tyrosine Phosphatase Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.495, 68.868, 58.701 |
| Unit cell angles | 90.00, 101.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cfv |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 16800 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 92.6 | |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291.15 | 2.0 M (NH4)2SO4, 0.1 M MES, 0.2 M Na/KPO4, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
