3GXU

Crystal structure of Eph receptor and ephrin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	54.651, 48.711, 64.469
Unit cell angles	90.00, 110.43, 90.00

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.273
Rwork	0.182
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ckh 1kgy
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	10938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	23.5% PEG4000, 0.1M Tris, 0.2M MgCl2, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K