3GMH
Crystal Structure of the Mad2 Dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-01-01 |
Detector | SBC-2 |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 124.829, 104.918, 157.746 |
Unit cell angles | 90.00, 97.57, 90.00 |
Refinement procedure
Resolution | 49.735 - 3.950 |
R-factor | 0.2199 |
Rwork | 0.218 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v64 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.023 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.090 |
High resolution limit [Å] | 3.950 | 3.950 |
Rmerge | 0.165 | 0.780 |
Number of reflections | 35718 | |
<I/σ(I)> | 11.6 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.6M ammonium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |