Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3GMH

Crystal Structure of the Mad2 Dimer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000070	biological_process	mitotic sister chromatid segregation
A	0000132	biological_process	establishment of mitotic spindle orientation
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000922	cellular_component	spindle pole
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0007049	biological_process	cell cycle
A	0007094	biological_process	mitotic spindle assembly checkpoint signaling
A	0033597	cellular_component	mitotic checkpoint complex
A	0042177	biological_process	negative regulation of protein catabolic process
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0043066	biological_process	negative regulation of apoptotic process
A	0044615	cellular_component	nuclear pore nuclear basket
A	0045930	biological_process	negative regulation of mitotic cell cycle
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0051301	biological_process	cell division
A	0051660	biological_process	establishment of centrosome localization
A	0072686	cellular_component	mitotic spindle
A	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
A	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
A	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
B	0000070	biological_process	mitotic sister chromatid segregation
B	0000132	biological_process	establishment of mitotic spindle orientation
B	0000775	cellular_component	chromosome, centromeric region
B	0000776	cellular_component	kinetochore
B	0000922	cellular_component	spindle pole
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005694	cellular_component	chromosome
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0007049	biological_process	cell cycle
B	0007094	biological_process	mitotic spindle assembly checkpoint signaling
B	0033597	cellular_component	mitotic checkpoint complex
B	0042177	biological_process	negative regulation of protein catabolic process
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0043066	biological_process	negative regulation of apoptotic process
B	0044615	cellular_component	nuclear pore nuclear basket
B	0045930	biological_process	negative regulation of mitotic cell cycle
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0051301	biological_process	cell division
B	0051660	biological_process	establishment of centrosome localization
B	0072686	cellular_component	mitotic spindle
B	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
B	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
B	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
C	0000070	biological_process	mitotic sister chromatid segregation
C	0000132	biological_process	establishment of mitotic spindle orientation
C	0000775	cellular_component	chromosome, centromeric region
C	0000776	cellular_component	kinetochore
C	0000922	cellular_component	spindle pole
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005694	cellular_component	chromosome
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0007049	biological_process	cell cycle
C	0007094	biological_process	mitotic spindle assembly checkpoint signaling
C	0033597	cellular_component	mitotic checkpoint complex
C	0042177	biological_process	negative regulation of protein catabolic process
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0043066	biological_process	negative regulation of apoptotic process
C	0044615	cellular_component	nuclear pore nuclear basket
C	0045930	biological_process	negative regulation of mitotic cell cycle
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0051301	biological_process	cell division
C	0051660	biological_process	establishment of centrosome localization
C	0072686	cellular_component	mitotic spindle
C	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
C	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
C	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
D	0000070	biological_process	mitotic sister chromatid segregation
D	0000132	biological_process	establishment of mitotic spindle orientation
D	0000775	cellular_component	chromosome, centromeric region
D	0000776	cellular_component	kinetochore
D	0000922	cellular_component	spindle pole
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0007049	biological_process	cell cycle
D	0007094	biological_process	mitotic spindle assembly checkpoint signaling
D	0033597	cellular_component	mitotic checkpoint complex
D	0042177	biological_process	negative regulation of protein catabolic process
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0043066	biological_process	negative regulation of apoptotic process
D	0044615	cellular_component	nuclear pore nuclear basket
D	0045930	biological_process	negative regulation of mitotic cell cycle
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0051301	biological_process	cell division
D	0051660	biological_process	establishment of centrosome localization
D	0072686	cellular_component	mitotic spindle
D	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
D	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
D	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
E	0000070	biological_process	mitotic sister chromatid segregation
E	0000132	biological_process	establishment of mitotic spindle orientation
E	0000775	cellular_component	chromosome, centromeric region
E	0000776	cellular_component	kinetochore
E	0000922	cellular_component	spindle pole
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0007049	biological_process	cell cycle
E	0007094	biological_process	mitotic spindle assembly checkpoint signaling
E	0033597	cellular_component	mitotic checkpoint complex
E	0042177	biological_process	negative regulation of protein catabolic process
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0043066	biological_process	negative regulation of apoptotic process
E	0044615	cellular_component	nuclear pore nuclear basket
E	0045930	biological_process	negative regulation of mitotic cell cycle
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0051301	biological_process	cell division
E	0051660	biological_process	establishment of centrosome localization
E	0072686	cellular_component	mitotic spindle
E	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
E	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
E	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
F	0000070	biological_process	mitotic sister chromatid segregation
F	0000132	biological_process	establishment of mitotic spindle orientation
F	0000775	cellular_component	chromosome, centromeric region
F	0000776	cellular_component	kinetochore
F	0000922	cellular_component	spindle pole
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005694	cellular_component	chromosome
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0007049	biological_process	cell cycle
F	0007094	biological_process	mitotic spindle assembly checkpoint signaling
F	0033597	cellular_component	mitotic checkpoint complex
F	0042177	biological_process	negative regulation of protein catabolic process
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0043066	biological_process	negative regulation of apoptotic process
F	0044615	cellular_component	nuclear pore nuclear basket
F	0045930	biological_process	negative regulation of mitotic cell cycle
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0051301	biological_process	cell division
F	0051660	biological_process	establishment of centrosome localization
F	0072686	cellular_component	mitotic spindle
F	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
F	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
F	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
G	0000070	biological_process	mitotic sister chromatid segregation
G	0000132	biological_process	establishment of mitotic spindle orientation
G	0000775	cellular_component	chromosome, centromeric region
G	0000776	cellular_component	kinetochore
G	0000922	cellular_component	spindle pole
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005694	cellular_component	chromosome
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0007049	biological_process	cell cycle
G	0007094	biological_process	mitotic spindle assembly checkpoint signaling
G	0033597	cellular_component	mitotic checkpoint complex
G	0042177	biological_process	negative regulation of protein catabolic process
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0043066	biological_process	negative regulation of apoptotic process
G	0044615	cellular_component	nuclear pore nuclear basket
G	0045930	biological_process	negative regulation of mitotic cell cycle
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0051301	biological_process	cell division
G	0051660	biological_process	establishment of centrosome localization
G	0072686	cellular_component	mitotic spindle
G	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
G	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
G	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
H	0000070	biological_process	mitotic sister chromatid segregation
H	0000132	biological_process	establishment of mitotic spindle orientation
H	0000775	cellular_component	chromosome, centromeric region
H	0000776	cellular_component	kinetochore
H	0000922	cellular_component	spindle pole
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005694	cellular_component	chromosome
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0007049	biological_process	cell cycle
H	0007094	biological_process	mitotic spindle assembly checkpoint signaling
H	0033597	cellular_component	mitotic checkpoint complex
H	0042177	biological_process	negative regulation of protein catabolic process
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0043066	biological_process	negative regulation of apoptotic process
H	0044615	cellular_component	nuclear pore nuclear basket
H	0045930	biological_process	negative regulation of mitotic cell cycle
H	0048471	cellular_component	perinuclear region of cytoplasm
H	0051301	biological_process	cell division
H	0051660	biological_process	establishment of centrosome localization
H	0072686	cellular_component	mitotic spindle
H	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
H	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
H	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
I	0000070	biological_process	mitotic sister chromatid segregation
I	0000132	biological_process	establishment of mitotic spindle orientation
I	0000775	cellular_component	chromosome, centromeric region
I	0000776	cellular_component	kinetochore
I	0000922	cellular_component	spindle pole
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005694	cellular_component	chromosome
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005856	cellular_component	cytoskeleton
I	0007049	biological_process	cell cycle
I	0007094	biological_process	mitotic spindle assembly checkpoint signaling
I	0033597	cellular_component	mitotic checkpoint complex
I	0042177	biological_process	negative regulation of protein catabolic process
I	0042802	molecular_function	identical protein binding
I	0042803	molecular_function	protein homodimerization activity
I	0043066	biological_process	negative regulation of apoptotic process
I	0044615	cellular_component	nuclear pore nuclear basket
I	0045930	biological_process	negative regulation of mitotic cell cycle
I	0048471	cellular_component	perinuclear region of cytoplasm
I	0051301	biological_process	cell division
I	0051660	biological_process	establishment of centrosome localization
I	0072686	cellular_component	mitotic spindle
I	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
I	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
I	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
J	0000070	biological_process	mitotic sister chromatid segregation
J	0000132	biological_process	establishment of mitotic spindle orientation
J	0000775	cellular_component	chromosome, centromeric region
J	0000776	cellular_component	kinetochore
J	0000922	cellular_component	spindle pole
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005694	cellular_component	chromosome
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005856	cellular_component	cytoskeleton
J	0007049	biological_process	cell cycle
J	0007094	biological_process	mitotic spindle assembly checkpoint signaling
J	0033597	cellular_component	mitotic checkpoint complex
J	0042177	biological_process	negative regulation of protein catabolic process
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0043066	biological_process	negative regulation of apoptotic process
J	0044615	cellular_component	nuclear pore nuclear basket
J	0045930	biological_process	negative regulation of mitotic cell cycle
J	0048471	cellular_component	perinuclear region of cytoplasm
J	0051301	biological_process	cell division
J	0051660	biological_process	establishment of centrosome localization
J	0072686	cellular_component	mitotic spindle
J	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
J	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
J	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
K	0000070	biological_process	mitotic sister chromatid segregation
K	0000132	biological_process	establishment of mitotic spindle orientation
K	0000775	cellular_component	chromosome, centromeric region
K	0000776	cellular_component	kinetochore
K	0000922	cellular_component	spindle pole
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005694	cellular_component	chromosome
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005856	cellular_component	cytoskeleton
K	0007049	biological_process	cell cycle
K	0007094	biological_process	mitotic spindle assembly checkpoint signaling
K	0033597	cellular_component	mitotic checkpoint complex
K	0042177	biological_process	negative regulation of protein catabolic process
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0043066	biological_process	negative regulation of apoptotic process
K	0044615	cellular_component	nuclear pore nuclear basket
K	0045930	biological_process	negative regulation of mitotic cell cycle
K	0048471	cellular_component	perinuclear region of cytoplasm
K	0051301	biological_process	cell division
K	0051660	biological_process	establishment of centrosome localization
K	0072686	cellular_component	mitotic spindle
K	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
K	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
K	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
L	0000070	biological_process	mitotic sister chromatid segregation
L	0000132	biological_process	establishment of mitotic spindle orientation
L	0000775	cellular_component	chromosome, centromeric region
L	0000776	cellular_component	kinetochore
L	0000922	cellular_component	spindle pole
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005694	cellular_component	chromosome
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005856	cellular_component	cytoskeleton
L	0007049	biological_process	cell cycle
L	0007094	biological_process	mitotic spindle assembly checkpoint signaling
L	0033597	cellular_component	mitotic checkpoint complex
L	0042177	biological_process	negative regulation of protein catabolic process
L	0042802	molecular_function	identical protein binding
L	0042803	molecular_function	protein homodimerization activity
L	0043066	biological_process	negative regulation of apoptotic process
L	0044615	cellular_component	nuclear pore nuclear basket
L	0045930	biological_process	negative regulation of mitotic cell cycle
L	0048471	cellular_component	perinuclear region of cytoplasm
L	0051301	biological_process	cell division
L	0051660	biological_process	establishment of centrosome localization
L	0072686	cellular_component	mitotic spindle
L	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
L	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
L	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 H 206

Chain	Residue
A	TYR33
A	ARG45
B	ARG45
H	TYR49
H	GLY50

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 L 206

Chain	Residue
C	TYR33
L	LYS48
L	TYR49
L	GLY50

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 D 206

Chain	Residue
D	LYS48
D	TYR49
D	GLY50
K	TYR33
K	ARG45
L	ARG45

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 206

Chain	Residue
B	LYS48
B	TYR49
B	GLY50
G	TYR33
G	ARG45
H	ARG45

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 206

Chain	Residue
A	ARG182
A	SER185
A	LYS192

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 206

Chain	Residue
C	ARG129
C	ARG133
D	ARG182

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 207

Chain	Residue
C	ARG182
C	SER185
C	LYS192

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 207

Chain	Residue
A	ARG129
A	ARG133
B	GLY36
B	ARG182

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 I 206

Chain	Residue
I	ARG129
I	ARG133
J	GLY36
J	ARG182

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 206

Chain	Residue
E	ARG129
E	ARG133
F	GLY36
F	ARG182

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 K 206

Chain	Residue
K	ARG129
K	ARG133
L	GLY36
L	LEU145
L	ARG182

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 G 206

Chain	Residue
G	ARG129
G	ARG133
H	GLY36
H	ARG182

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER130
E	SER185
F	SER130
F	SER185
G	SER130
G	SER185
H	SER130
H	SER185
I	SER130
I	SER185
J	SER130
A	SER185
J	SER185
K	SER130
K	SER185
L	SER130
L	SER185
B	SER130
B	SER185
C	SER130
C	SER185
D	SER130
D	SER185
E	SER130

site_id	SWS_FT_FI2
Number of Residues	24
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:12574116

Chain	Residue	Details
A	SER170
E	SER178
F	SER170
F	SER178
G	SER170
G	SER178
H	SER170
H	SER178
I	SER170
I	SER178
J	SER170
A	SER178
J	SER178
K	SER170
K	SER178
L	SER170
L	SER178
B	SER170
B	SER178
C	SER170
C	SER178
D	SER170
D	SER178
E	SER170

site_id	SWS_FT_FI3
Number of Residues	12
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:12574116, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER195
J	SER195
K	SER195
L	SER195
B	SER195
C	SER195
D	SER195
E	SER195
F	SER195
G	SER195
H	SER195
I	SER195

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)