3GMH

Crystal Structure of the Mad2 Dimer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000922	cellular_component	spindle pole
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0007094	biological_process	mitotic spindle assembly checkpoint signaling
A	0033597	cellular_component	mitotic checkpoint complex
A	0042177	biological_process	negative regulation of protein catabolic process
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0044615	cellular_component	nuclear pore nuclear basket
A	0045930	biological_process	negative regulation of mitotic cell cycle
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0051301	biological_process	cell division
A	0051783	biological_process	regulation of nuclear division
A	0072686	cellular_component	mitotic spindle
A	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
A	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
A	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
A	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
B	0000775	cellular_component	chromosome, centromeric region
B	0000776	cellular_component	kinetochore
B	0000922	cellular_component	spindle pole
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005694	cellular_component	chromosome
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0007094	biological_process	mitotic spindle assembly checkpoint signaling
B	0033597	cellular_component	mitotic checkpoint complex
B	0042177	biological_process	negative regulation of protein catabolic process
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0044615	cellular_component	nuclear pore nuclear basket
B	0045930	biological_process	negative regulation of mitotic cell cycle
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0051301	biological_process	cell division
B	0051783	biological_process	regulation of nuclear division
B	0072686	cellular_component	mitotic spindle
B	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
B	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
B	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
B	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
C	0000775	cellular_component	chromosome, centromeric region
C	0000776	cellular_component	kinetochore
C	0000922	cellular_component	spindle pole
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005694	cellular_component	chromosome
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0007094	biological_process	mitotic spindle assembly checkpoint signaling
C	0033597	cellular_component	mitotic checkpoint complex
C	0042177	biological_process	negative regulation of protein catabolic process
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0044615	cellular_component	nuclear pore nuclear basket
C	0045930	biological_process	negative regulation of mitotic cell cycle
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0051301	biological_process	cell division
C	0051783	biological_process	regulation of nuclear division
C	0072686	cellular_component	mitotic spindle
C	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
C	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
C	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
C	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
D	0000775	cellular_component	chromosome, centromeric region
D	0000776	cellular_component	kinetochore
D	0000922	cellular_component	spindle pole
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0007094	biological_process	mitotic spindle assembly checkpoint signaling
D	0033597	cellular_component	mitotic checkpoint complex
D	0042177	biological_process	negative regulation of protein catabolic process
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0044615	cellular_component	nuclear pore nuclear basket
D	0045930	biological_process	negative regulation of mitotic cell cycle
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0051301	biological_process	cell division
D	0051783	biological_process	regulation of nuclear division
D	0072686	cellular_component	mitotic spindle
D	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
D	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
D	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
D	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
E	0000775	cellular_component	chromosome, centromeric region
E	0000776	cellular_component	kinetochore
E	0000922	cellular_component	spindle pole
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0007094	biological_process	mitotic spindle assembly checkpoint signaling
E	0033597	cellular_component	mitotic checkpoint complex
E	0042177	biological_process	negative regulation of protein catabolic process
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0044615	cellular_component	nuclear pore nuclear basket
E	0045930	biological_process	negative regulation of mitotic cell cycle
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0051301	biological_process	cell division
E	0051783	biological_process	regulation of nuclear division
E	0072686	cellular_component	mitotic spindle
E	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
E	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
E	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
E	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
F	0000775	cellular_component	chromosome, centromeric region
F	0000776	cellular_component	kinetochore
F	0000922	cellular_component	spindle pole
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005694	cellular_component	chromosome
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0007094	biological_process	mitotic spindle assembly checkpoint signaling
F	0033597	cellular_component	mitotic checkpoint complex
F	0042177	biological_process	negative regulation of protein catabolic process
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0044615	cellular_component	nuclear pore nuclear basket
F	0045930	biological_process	negative regulation of mitotic cell cycle
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0051301	biological_process	cell division
F	0051783	biological_process	regulation of nuclear division
F	0072686	cellular_component	mitotic spindle
F	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
F	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
F	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
F	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
G	0000775	cellular_component	chromosome, centromeric region
G	0000776	cellular_component	kinetochore
G	0000922	cellular_component	spindle pole
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005694	cellular_component	chromosome
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0007094	biological_process	mitotic spindle assembly checkpoint signaling
G	0033597	cellular_component	mitotic checkpoint complex
G	0042177	biological_process	negative regulation of protein catabolic process
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0044615	cellular_component	nuclear pore nuclear basket
G	0045930	biological_process	negative regulation of mitotic cell cycle
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0051301	biological_process	cell division
G	0051783	biological_process	regulation of nuclear division
G	0072686	cellular_component	mitotic spindle
G	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
G	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
G	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
G	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
H	0000775	cellular_component	chromosome, centromeric region
H	0000776	cellular_component	kinetochore
H	0000922	cellular_component	spindle pole
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005694	cellular_component	chromosome
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0007094	biological_process	mitotic spindle assembly checkpoint signaling
H	0033597	cellular_component	mitotic checkpoint complex
H	0042177	biological_process	negative regulation of protein catabolic process
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0044615	cellular_component	nuclear pore nuclear basket
H	0045930	biological_process	negative regulation of mitotic cell cycle
H	0048471	cellular_component	perinuclear region of cytoplasm
H	0051301	biological_process	cell division
H	0051783	biological_process	regulation of nuclear division
H	0072686	cellular_component	mitotic spindle
H	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
H	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
H	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
H	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
I	0000775	cellular_component	chromosome, centromeric region
I	0000776	cellular_component	kinetochore
I	0000922	cellular_component	spindle pole
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005694	cellular_component	chromosome
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0007094	biological_process	mitotic spindle assembly checkpoint signaling
I	0033597	cellular_component	mitotic checkpoint complex
I	0042177	biological_process	negative regulation of protein catabolic process
I	0042802	molecular_function	identical protein binding
I	0042803	molecular_function	protein homodimerization activity
I	0044615	cellular_component	nuclear pore nuclear basket
I	0045930	biological_process	negative regulation of mitotic cell cycle
I	0048471	cellular_component	perinuclear region of cytoplasm
I	0051301	biological_process	cell division
I	0051783	biological_process	regulation of nuclear division
I	0072686	cellular_component	mitotic spindle
I	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
I	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
I	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
I	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
J	0000775	cellular_component	chromosome, centromeric region
J	0000776	cellular_component	kinetochore
J	0000922	cellular_component	spindle pole
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005694	cellular_component	chromosome
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0007094	biological_process	mitotic spindle assembly checkpoint signaling
J	0033597	cellular_component	mitotic checkpoint complex
J	0042177	biological_process	negative regulation of protein catabolic process
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0044615	cellular_component	nuclear pore nuclear basket
J	0045930	biological_process	negative regulation of mitotic cell cycle
J	0048471	cellular_component	perinuclear region of cytoplasm
J	0051301	biological_process	cell division
J	0051783	biological_process	regulation of nuclear division
J	0072686	cellular_component	mitotic spindle
J	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
J	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
J	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
J	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
K	0000775	cellular_component	chromosome, centromeric region
K	0000776	cellular_component	kinetochore
K	0000922	cellular_component	spindle pole
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005694	cellular_component	chromosome
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0007094	biological_process	mitotic spindle assembly checkpoint signaling
K	0033597	cellular_component	mitotic checkpoint complex
K	0042177	biological_process	negative regulation of protein catabolic process
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0044615	cellular_component	nuclear pore nuclear basket
K	0045930	biological_process	negative regulation of mitotic cell cycle
K	0048471	cellular_component	perinuclear region of cytoplasm
K	0051301	biological_process	cell division
K	0051783	biological_process	regulation of nuclear division
K	0072686	cellular_component	mitotic spindle
K	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
K	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
K	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
K	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex
L	0000775	cellular_component	chromosome, centromeric region
L	0000776	cellular_component	kinetochore
L	0000922	cellular_component	spindle pole
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005694	cellular_component	chromosome
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0007094	biological_process	mitotic spindle assembly checkpoint signaling
L	0033597	cellular_component	mitotic checkpoint complex
L	0042177	biological_process	negative regulation of protein catabolic process
L	0042802	molecular_function	identical protein binding
L	0042803	molecular_function	protein homodimerization activity
L	0044615	cellular_component	nuclear pore nuclear basket
L	0045930	biological_process	negative regulation of mitotic cell cycle
L	0048471	cellular_component	perinuclear region of cytoplasm
L	0051301	biological_process	cell division
L	0051783	biological_process	regulation of nuclear division
L	0072686	cellular_component	mitotic spindle
L	0090267	biological_process	positive regulation of mitotic cell cycle spindle assembly checkpoint
L	1901991	biological_process	negative regulation of mitotic cell cycle phase transition
L	1904667	biological_process	negative regulation of ubiquitin protein ligase activity
L	1990728	cellular_component	mitotic spindle assembly checkpoint MAD1-MAD2 complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 H 206

Chain	Residue
A	TYR33
A	ARG45
B	ARG45
H	TYR49
H	GLY50

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 L 206

Chain	Residue
C	TYR33
L	LYS48
L	TYR49
L	GLY50

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 D 206

Chain	Residue
D	LYS48
D	TYR49
D	GLY50
K	TYR33
K	ARG45
L	ARG45

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 206

Chain	Residue
B	LYS48
B	TYR49
B	GLY50
G	TYR33
G	ARG45
H	ARG45

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 206

Chain	Residue
A	ARG182
A	SER185
A	LYS192

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 206

Chain	Residue
C	ARG129
C	ARG133
D	ARG182

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 207

Chain	Residue
C	ARG182
C	SER185
C	LYS192

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 207

Chain	Residue
A	ARG129
A	ARG133
B	GLY36
B	ARG182

---

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 I 206

Chain	Residue
I	ARG129
I	ARG133
J	GLY36
J	ARG182

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 206

Chain	Residue
E	ARG129
E	ARG133
F	GLY36
F	ARG182

---

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 K 206

Chain	Residue
K	ARG129
K	ARG133
L	GLY36
L	LEU145
L	ARG182

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 G 206

Chain	Residue
G	ARG129
G	ARG133
H	GLY36
H	ARG182

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	366
Details	Domain: {"description":"HORMA","evidences":[{"source":"PROSITE-ProRule","id":"PRU00109","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"12574116","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"12574116","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---