3GIZ
Crystal structure of the Fab fragment of anti-CD20 antibody Ofatumumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.330, 55.944, 79.780 |
| Unit cell angles | 90.00, 122.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.19752 |
| Rwork | 0.195 |
| R-free | 0.24957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u6a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.375 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.325 |
| Number of reflections | 26051 | |
| <I/σ(I)> | 7 | 2.1 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 0.2 M zinc acetate, 20% PEG3350, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
