3GAD
Structure of apomif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.880, 98.940, 108.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.070 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mfi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.102 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.510 | 1.900 | |
| High resolution limit [Å] | 1.800 | 10.000 | 1.800 |
| Rmerge | 0.038 | 0.014 | 0.219 |
| Number of reflections | 92291 | 597 | 13541 |
| <I/σ(I)> | 80.8 | 7.5 | |
| Completeness [%] | 98.0 | 93.4 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.6 | 289 | 2.5M Ammonium sulfate, 0.1M Sodium acetate trihydrate pH4.6, hanging drop, temperature 289K |
