3G8Q
A cytidine deaminase edits C-to-U in transfer RNAs in archaea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-25 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.465, 77.060, 109.082 |
Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.20496 |
Rwork | 0.202 |
R-free | 0.26482 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.217 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 55130 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 295 | 0.1 M Na cacodylate, 32-40% PEG 400, 0.1 M Ca acetate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |