3G7V

Islet Amyloid Polypeptide (IAPP or Amylin) fused to Maltose Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	104.025, 96.407, 103.996
Unit cell angles	90.00, 90.53, 90.00

Refinement procedure

Resolution	73.920 - 1.860
R-factor	0.178
Rwork	0.176
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mpd
RMSD bond length	0.012
RMSD bond angle	1.313
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	1.920
High resolution limit [Å]	1.850	3.990	1.850
Rmerge	0.054	0.035	0.460
Number of reflections	169517
<I/σ(I)>	27.432
Completeness [%]	98.4	99.6	96.9
Redundancy	5.5	5.6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2.0 M Ammonium Sulfate, 0.1M Sodium Acetate pH 4.6, vapor diffusion, hanging drop, temperature 298K