3G6H
Src Thr338Ile inhibited in the DFG-Asp-Out conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.938, 63.344, 74.179 |
| Unit cell angles | 100.99, 89.39, 90.20 |
Refinement procedure
| Resolution | 34.790 - 2.350 |
| R-factor | 0.22781 |
| Rwork | 0.225 |
| R-free | 0.28268 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 28194 | |
| Completeness [%] | 86.7 | 31.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES pH 6.0, 8 % PEG 3350, 3 % Glycerol, 45 mM sodium acetate, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
