3G3B
Structure of a lamprey variable lymphocyte receptor mutant in complex with a protein antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.874, 106.244, 218.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.24426 |
| Rwork | 0.242 |
| R-free | 0.28612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g3a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.418 |
| Number of reflections | 60645 | |
| <I/σ(I)> | 29.1 | 6.7 |
| Redundancy | 13.6 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 296 | 8% (w/v) polyethylene glycol 8000, 0.1 M Tris-HCl, pH 8.5, EVAPORATION, temperature 296K |
