3G33
Crystal structure of CDK4/cyclin D3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-17 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 0.97650 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 141.297, 141.297, 143.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.190 - 3.000 |
R-factor | 0.28304 |
Rwork | 0.281 |
R-free | 0.31442 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.535 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.830 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.067 | 0.507 |
Number of reflections | 29147 | |
<I/σ(I)> | 13.8 | 1.3 |
Completeness [%] | 98.4 | 94.9 |
Redundancy | 3.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | 0.1 M Hepes, 15% PEG 3,350, 10% tacsimate, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |