3G1N
Catalytic domain of the human E3 ubiquitin-protein ligase HUWE1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.530, 72.213, 77.238 |
| Unit cell angles | 90.00, 106.94, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.600 |
| R-factor | 0.21726 |
| Rwork | 0.214 |
| R-free | 0.27919 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nd7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 28536 | |
| <I/σ(I)> | 10.851 | 1.96 |
| Completeness [%] | 98.9 | 91.8 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15% PEG-4000, 10% ISOPROPANOL, 0.1 M TRIS BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
