3FUB
Crystal structure of GDNF-GFRalpha1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.933 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 45.200, 84.100, 179.200 |
Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.350 |
R-factor | 0.22834 |
Rwork | 0.226 |
R-free | 0.28021 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v5e |
RMSD bond length | 0.007 |
RMSD bond angle | 1.091 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.450 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 30497 | |
<I/σ(I)> | 2.95 | |
Completeness [%] | 97.0 | 91.3 |
Redundancy | 3.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | 15% PEG4000, 0.15 M (NH4)2SO4, 0.1 M MES buffer pH 6, Vapor diffusion, temperature 293K |