3FSL

Crystal structure of tyrosine aminotransferase tripple mutant (P181Q,R183G,A321K) from Escherichia coli at 2.35 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	294
Detector technology	IMAGE PLATE
Collection date	1996-10-07
Detector	MAR scanner 180 mm plate
Wavelength(s)	1.02
Spacegroup name	P 21 21 21
Unit cell lengths	98.880, 119.160, 242.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.350
R-factor	0.215
Rwork	0.213
R-free	0.26000
RMSD bond length	0.021
RMSD bond angle	1.758
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Rmerge	0.080
Number of reflections	119658
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	15-17% PEG4000, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K