3FRT

The structure of human CHMP3 (residues 8 - 222).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-03-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97607
Spacegroup name	P 1 21 1
Unit cell lengths	36.472, 131.514, 48.508
Unit cell angles	90.00, 108.06, 90.00

Refinement procedure

Resolution	30.000 - 4.000
R-factor	0.388
Rwork	0.383
R-free	0.43600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gd5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.140
High resolution limit [Å]	4.000	4.000
Rmerge	0.160	0.439
Number of reflections	2950
<I/σ(I)>	9.33	2.92
Completeness [%]	78.7	78.7
Redundancy	4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	286	10-22% PEG 6000 0.1 M Hepes, pH 7.0-8.0, VAPOR DIFFUSION, SITTING DROP, temperature 286K