3FMZ

Crystal Structure of Retinol-Binding Protein 4 (RBP4) in complex with non-retinoid ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999
Spacegroup name	P 65
Unit cell lengths	84.802, 84.802, 119.557
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.226
Rwork	0.223
R-free	0.28600
RMSD bond length	0.006
RMSD bond angle	1.148
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.521	119.560	3.060
High resolution limit [Å]	2.900	9.170	2.900
Rmerge	0.125	0.043	0.402
Total number of observations		1312	6012
Number of reflections	10890
<I/σ(I)>	5.773	13.4	1.9
Completeness [%]	100.0	100	100
Redundancy	3.7	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	289	0.4M Mg formate, 0.1M GndHCl, 0.1M Bis-Tris 6.5, vapor diffusion, temperature 289K