3FGU
Catalytic complex of Human Glucokinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-11-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0001 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.880, 82.120, 86.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
R-factor | 0.19977 |
Rwork | 0.197 |
R-free | 0.25529 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f9m |
RMSD bond length | 0.011 |
RMSD bond angle | 1.267 |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.076 | 0.330 |
Number of reflections | 22231 | |
<I/σ(I)> | 17.2 | 3.1 |
Completeness [%] | 84.5 | 84.8 |
Redundancy | 3.6 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |