3FGU
Catalytic complex of Human Glucokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-11-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.880, 82.120, 86.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.19977 |
| Rwork | 0.197 |
| R-free | 0.25529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f9m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.267 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.076 | 0.330 |
| Number of reflections | 22231 | |
| <I/σ(I)> | 17.2 | 3.1 |
| Completeness [%] | 84.5 | 84.8 |
| Redundancy | 3.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
