3FEX
Crystal structure of the CBC-importin alpha complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.508, 102.051, 107.878 |
| Unit cell angles | 90.00, 108.82, 90.00 |
Refinement procedure
| Resolution | 15.652 - 3.549 |
| R-factor | 0.2212 |
| Rwork | 0.217 |
| R-free | 0.29240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fey |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.549 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.652 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.161 | 0.428 |
| Number of reflections | 18110 | |
| <I/σ(I)> | 12.3 | 2.58 |
| Completeness [%] | 91.4 | 92.5 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM KCl, 12% PEG 8000, 100 mM Tris-HCl pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
