3FEX
Crystal structure of the CBC-importin alpha complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.508, 102.051, 107.878 |
Unit cell angles | 90.00, 108.82, 90.00 |
Refinement procedure
Resolution | 15.652 - 3.549 |
R-factor | 0.2212 |
Rwork | 0.217 |
R-free | 0.29240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fey |
RMSD bond length | 0.005 |
RMSD bond angle | 0.549 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.652 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.161 | 0.428 |
Number of reflections | 18110 | |
<I/σ(I)> | 12.3 | 2.58 |
Completeness [%] | 91.4 | 92.5 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM KCl, 12% PEG 8000, 100 mM Tris-HCl pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |