3FCS
Structure of complete ectodomain of integrin aIIBb3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 41 |
Unit cell lengths | 81.300, 81.300, 654.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.310 - 2.550 |
R-factor | 0.23332 |
Rwork | 0.233 |
R-free | 0.26802 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Combination of PDB ENTRY 1JV2 and 1TXV |
RMSD bond length | 0.003 |
RMSD bond angle | 0.741 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.071 | 0.566 |
Number of reflections | 135066 | |
<I/σ(I)> | 12.17 | 2.13 |
Completeness [%] | 98.6 | 93.9 |
Redundancy | 4.55 | 3.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 10% PEG 3350, 50 mM magnesium acetate, and 0.1 M imidazole, pH 7.0, VAPOR DIFFUSION, temperature 298.0K |