3FC9
Crystal structure of the Mimivirus NDK +Kpn-N62L double mutant complexed with CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-08-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.586, 153.043, 184.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.467 - 2.800 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.079 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 26346 | |
| <I/σ(I)> | 6.5 | 2.5 |
| Completeness [%] | 91.0 | 92.4 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40-44% MPD, 0.1M MOPS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
