3FAV
Structure of the CFP10-ESAT6 complex from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.340, 23.930, 83.860 |
| Unit cell angles | 90.00, 94.36, 90.00 |
Refinement procedure
| Resolution | 19.858 - 2.150 |
| Rwork | 0.199 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wa8 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.055 | 0.197 |
| Number of reflections | 17336 | 978 |
| <I/σ(I)> | 14.4 | 4.2 |
| Completeness [%] | 95.5 | 73.4 |
| Redundancy | 3.35 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 33% (v/v) PEG 600, 200mM zinc acetate, 100mM imidazol pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
