1WA8

Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria

Summary for 1WA8

• Entry DOI: 10.2210/pdb1wa8/pdb
• Descriptor: ESAT-6 LIKE PROTEIN ESXB, 6 KDA EARLY SECRETORY ANTIGENIC TARGET (ESAT-6) (2 entities in total)
• Functional Keywords: tuberculosis, cfp-10, esat-6, helix-turn-helix, four helix bundle, mycobacteria, pathogenesis, solution structure, psi, protein structure initiative, tb structural genomics consortium, tbsgc
• Biological source: MYCOBACTERIUM BOVIS; More
• Total number of polymer chains: 2
• Total formula weight: 20580.35

Authors

Renshaw, P.S.,Lightbody, K.L.,Veverka, V.,Muskett, F.W.,Kelly, G.,Frenkiel, T.A.,Gordon, S.V.,Hewinson, R.G.,Burke, B.,Norman, J.,Williamson, R.A.,Carr, M.D.,TB Structural Genomics Consortium (TBSGC) (deposition date: 2004-10-25, release date: 2005-06-27, Last modification date: 2024-05-15)

Primary citation

Renshaw, P.S.,Lightbody, K.L.,Veverka, V.,Muskett, F.W.,Kelly, G.,Frenkiel, T.A.,Gordon, S.V.,Hewinson, R.G.,Burke, B.,Norman, J.,Williamson, R.A.,Carr, M.D.
Structure and Function of the Complex Formed by the Tuberculosis Virulence Factors Cfp-10 and Esat-6
Embo J., 24:2491-, 2005

PubMed Abstract: The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.

PubMed: 15973432
DOI: 10.1038/SJ.EMBOJ.7600732

Experimental method

SOLUTION NMR