3F73
Alignment of guide-target seed duplex within an argonaute silencing complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.162, 120.527, 109.085 |
| Unit cell angles | 90.00, 105.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.28200 |
| Starting model (for MR) | 3dlh |
| RMSD bond length | 0.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.390 | |
| Number of reflections | 29830 | |
| <I/σ(I)> | 15.8 | 2.1 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 308 | 10% (v/v) PEG400, 50mM Mes 5.6, 0.1M KCl, 15 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 308K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | KCL | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 2 | PEG 400 | ||
| 6 | 1 | 2 | MES | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | MgCl2 |
