3F6K
Crystal structure of the Vps10p domain of human sortilin/NTS3 in complex with neurotensin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95008 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.760, 74.530, 108.330 |
| Unit cell angles | 90.00, 131.87, 90.00 |
Refinement procedure
| Resolution | 40.320 - 2.000 |
| R-factor | 0.20524 |
| Rwork | 0.204 |
| R-free | 0.22879 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.832 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.440 |
| Number of reflections | 56985 | |
| <I/σ(I)> | 11.59 | 4.32 |
| Completeness [%] | 96.9 | 98.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 19% PEG 6000, 600 mM NaCl, 3% Glycerol, 100 mM HEPES, 93 mM Tris-HCl, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
