3F4B
Crystal structure of Plasmodium berghei Enoyl-acyl-carrier-protein reductase with TRICLOSAN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.339, 121.200, 87.832 |
| Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
| Resolution | 36.330 - 2.490 |
| R-factor | 0.1835 |
| Rwork | 0.183 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nhg |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.330 |
| High resolution limit [Å] | 2.490 |
| Number of reflections | 41328 |
| Completeness [%] | 95.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 289 | 100mM Tris pH 7.1, 14% PEG2000, 100mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
