3F1O
Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with an internally-bound artificial ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.761, 83.019, 41.553 |
| Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
| Resolution | 26.930 - 1.598 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b02 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.048 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.930 | 1.610 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.488 |
| Number of reflections | 31184 | |
| <I/σ(I)> | 23.5 | 2.1 |
| Completeness [%] | 98.4 | 97.7 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion combined with microseeding | 6.5 | 293 | 30% PEG 3350, 0.1M BisTris, 0.05M Tris, 0.017M NaCl, 0.005M DTT , pH 6.5, vapor diffusion combined with microseeding, temperature 293K |
