3F1N
Crystal structure of a high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with internally bound ethylene glycol.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-13 |
Detector | SBC-2 |
Wavelength(s) | 0.97924 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 73.321, 82.908, 41.400 |
Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
Resolution | 30.972 - 1.479 |
R-factor | 0.173 |
Rwork | 0.172 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b02 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.226 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 1.490 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.042 | 0.045 |
Number of reflections | 39281 | |
<I/σ(I)> | 32.6 | 1.9 |
Completeness [%] | 99.1 | 91.3 |
Redundancy | 4.8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion combined with microseeding | 6.5 | 293 | 30% PEG 3350, 0.1M BisTris, 0.05M Tris, 0.017M NaCl, 0.005M DTT, pH 6.5, vapor diffusion combined with microseeding, temperature 293K |