3EZ3
Crystal Structure of Plasmodium vivax geranylgeranylpyrophosphate synthase PVX_092040 with zoledronate and IPP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9197 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.266, 109.696, 139.529 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.430 - 2.304 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.649 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.490 |
High resolution limit [Å] | 2.304 | 2.304 |
Rmerge | 0.184 | |
Number of reflections | 72515 | |
<I/σ(I)> | 10.667 | 1.4 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.3 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20 % PEG3350 0.2 M Li2SO4 0.1 M Tris 8.5 5% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |