3EPI
Structure of Human DNA Polymerase Iota complexed with N2-ethylguanine and incoming TTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-30 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.643, 98.643, 202.231 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.990 - 2.900 |
R-factor | 0.23858 |
Rwork | 0.236 |
R-free | 0.28203 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ALZ minus DNA |
RMSD bond length | 0.010 |
RMSD bond angle | 1.532 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.990 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.144 | 0.323 |
Number of reflections | 13417 | |
<I/σ(I)> | 11.8 | 4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 27.44 | 26.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 12-15% PEG MME 5000, 0.2-0.4M Ammonium Sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG MME 5000 | ||
2 | 1 | 1 | Ammonium Sulfate | ||
3 | 1 | 1 | MES | ||
4 | 1 | 2 | PEG MME 5000 | ||
5 | 1 | 2 | Ammonium Sulfate | ||
6 | 1 | 2 | MES |