3EMW
Crystal Structure of human splA/ryanodine receptor domain and SOCS box containing 2 (SPSB2) in complex with a 20-residue VASA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.975653 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.470, 61.963, 118.581 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.830 - 1.800 |
R-factor | 0.17995 |
Rwork | 0.178 |
R-free | 0.21593 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jk9 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.548 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.830 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.078 | 0.618 |
Number of reflections | 24366 | |
<I/σ(I)> | 15 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.9 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.178M Na/KPO4, 17.8% PEG3350, 8.88% EtGly, pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |