3EI4
Structure of the hsDDB1-hsDDB2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.038 |
Spacegroup name | H 3 2 |
Unit cell lengths | 268.500, 268.500, 471.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.300 |
R-factor | 0.255 |
Rwork | 0.254 |
R-free | 0.28800 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.459 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.420 |
High resolution limit [Å] | 3.200 | 3.300 |
Rmerge | 0.142 | 0.549 |
Number of reflections | 85881 | |
<I/σ(I)> | 7.537 | 1.7 |
Completeness [%] | 86.1 | 19 |
Redundancy | 5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 200 mM (NH4)2SO4; 800 mM LiSO2; 100 mM Na-Citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |