3EI4
Structure of the hsDDB1-hsDDB2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.038 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 268.500, 268.500, 471.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.300 |
| R-factor | 0.255 |
| Rwork | 0.254 |
| R-free | 0.28800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.459 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.300 |
| Rmerge | 0.142 | 0.549 |
| Number of reflections | 85881 | |
| <I/σ(I)> | 7.537 | 1.7 |
| Completeness [%] | 86.1 | 19 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 200 mM (NH4)2SO4; 800 mM LiSO2; 100 mM Na-Citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
