3EHS
Crystal structure of the extracellular domain of human corticotropin releasing factor receptor type 1 (CRFR1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.018, 112.018, 145.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.760 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c4m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.233 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.059 | 0.573 |
| Number of reflections | 24253 | |
| <I/σ(I)> | 33.26 | 2.99 |
| Completeness [%] | 98.4 | 90.2 |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.7 | 293 | NaCl, sucrose, sodium acetate, pH 4.7, vapor diffusion, temperature 293K |
