3EHS
Crystal structure of the extracellular domain of human corticotropin releasing factor receptor type 1 (CRFR1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 112.018, 112.018, 145.786 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.760 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c4m |
RMSD bond length | 0.011 |
RMSD bond angle | 1.233 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.059 | 0.573 |
Number of reflections | 24253 | |
<I/σ(I)> | 33.26 | 2.99 |
Completeness [%] | 98.4 | 90.2 |
Redundancy | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.7 | 293 | NaCl, sucrose, sodium acetate, pH 4.7, vapor diffusion, temperature 293K |