3EH0
Crystal Structure of LpxD from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.950, 94.170, 103.660 |
| Unit cell angles | 90.00, 126.50, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.21689 |
| Rwork | 0.214 |
| R-free | 0.27911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model based on pdb code 2IUA |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.114 | 0.440 |
| Number of reflections | 36863 | |
| <I/σ(I)> | 10.6 | 3.1 |
| Completeness [%] | 96.7 | 97.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 290 | 0.1 M MES, 2.0 M (NH4)2SO4, 1% doixane, 1 mM DTT, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
